Light sheet fluorescence microscopy with active optical manipulation

Jun Yin; Liang Ruijing; Lingyao Yu

doi:10.1117/12.2687106

29 November 2023 Light sheet fluorescence microscopy with active optical manipulation

Jun Yin, Liang Ruijing, Lingyao Yu

Proceedings Volume 12767, Optoelectronic Imaging and Multimedia Technology X; 1276717 (2023) https://doi.org/10.1117/12.2687106
Event: SPIE/COS Photonics Asia, 2023, Beijing, China

Conference Poster

Abstract

We propose a light-sheet fluorescence microscopy imaging method based on active optical manipulation with linear laser optical tweezers. In this imaging method, a column lens is utilized to generate an excitation light sheet, and a linear laser light tweezer is adopted to capture and precisely control the rotation of the experimental object. The linear laser optical tweezers are used to capture and precisely control the rotation of the experimental object. The plane of the light sheet is perpendicular to the rotational plane of the experimental object, and the rotation of the experimental object at different angles can be controlled to realize the light sheet on the experimental object layer. The light sheet is perpendicular to the rotational plane of the subject. Most importantly, compared to traditional microscopy methods, this solution directly manipulates and observes the experimental object, which reduces the risk of the object changing position in the sample container. It reduces the uncertainty and error of image acquisition and reconstruction caused by the change of the position of the experimental object in the sample container.

Citation Download Citation

Jun Yin, Liang Ruijing, and Lingyao Yu "Light sheet fluorescence microscopy with active optical manipulation", Proc. SPIE 12767, Optoelectronic Imaging and Multimedia Technology X, 1276717 (29 November 2023); https://doi.org/10.1117/12.2687106

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