Label-free light scattering imaging of nanoscale small extracellular vesicles

Faihaa Mohammed Eltigani; Zhuo Wang; Qiao Liu; Shuanglian Wang; Xuantao Su

doi:10.1117/12.3001048

12 March 2024 Label-free light scattering imaging of nanoscale small extracellular vesicles

Faihaa Mohammed Eltigani, Zhuo Wang, Qiao Liu, Shuanglian Wang, Xuantao Su

Proceedings Volume 12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII; 1284605 (2024) https://doi.org/10.1117/12.3001048
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Small extracellular vesicles (sEVs), which are nanoparticles around 100 nm, have been widely studied in recent years in many interesting areas, such as cancer detection and drug delivery. Bulk analysis of extracellular vesicles provides average information about the EV population. However, single EV characterization enables a profound understanding of the biophysical properties of EV subpopulations, establishing an insightful view of the EVs function and composition. It is worth to explore light scattering imaging method for the analysis of single sEVs. We introduce here the deep-learning-based light scattering imaging method for analyzing label-free sEVs (DeepEVAnalyzer), which has been applied to measure the size of single sEVs. We also report our recent development of a light scattering imaging method to address the inverse problem, which is demonstrated to differentiate the label-free sEVs from healthy mice and those injected with malignant cells. Light scattering imaging together with machine learning for sEVs analysis may have potential diagnostic and therapeutic applications.

Citation Download Citation

Faihaa Mohammed Eltigani, Zhuo Wang, Qiao Liu, Shuanglian Wang, and Xuantao Su "Label-free light scattering imaging of nanoscale small extracellular vesicles", Proc. SPIE 12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII, 1284605 (12 March 2024); https://doi.org/10.1117/12.3001048

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Light scattering

Nanoparticles

Analytical research

Biomedical optics

Deep learning

Inverse scattering problem

Quantitative analysis

Show All Keywords

Keywords/Phrases

Search In:

Publication Years