Imaging microplastics with multiphoton tomographs

Vidyalakshmi Anilkumar; Aisada König; Karsten König

doi:10.1117/12.3000160

12 March 2024 Imaging microplastics with multiphoton tomographs

Vidyalakshmi Anilkumar, Aisada König, Karsten König

Author Affiliations +

Proceedings Volume 12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; 1284709 (2024) https://doi.org/10.1117/12.3000160
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Microplastic studies are crucial due to their impact on human health and the environment. However, the lack of a standardized method for microplastic identification and quantification hinders comprehensive analysis. Our research addresses these challenges by employing the advanced imaging technique Multimodal Multiphoton Tomography (MPT) including two-photon autofluorescence (AF), fluorescence lifetime imaging (FLIM) with phasor analysis, second harmonics generation (SHG), and reflectance confocal microscopy (RCM) with 50/80 MHz femtosecond laser pulses. The combined imaging approach allows also tracking of cosmetics with microplastics in-vivo in the human skin.

Conference Presentation

Citation Download Citation

Vidyalakshmi Anilkumar, Aisada König, and Karsten König "Imaging microplastics with multiphoton tomographs", Proc. SPIE 12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, 1284709 (12 March 2024); https://doi.org/10.1117/12.3000160

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