Exploring metal-molecule-metal nanoparticles (MMNP) configuration for introducing specificity in SPR biosensors

Aditi Chopra; Girish C. Mohanta; Sudipta Sarkar Pal

doi:10.1117/12.3021750

28 May 2024 Exploring metal-molecule-metal nanoparticles (MMNP) configuration for introducing specificity in SPR biosensors

Aditi Chopra, Girish C. Mohanta, Sudipta Sarkar Pal

Author Affiliations +

Proceedings Volume 13083, SPIE Future Sensing Technologies 2024; 130830P (2024) https://doi.org/10.1117/12.3021750
Event: SPIE Future Sensing Technologies, 2024, Yokohama, Japan

Abstract

We developed a “Metal-Molecule-Metal Nanoparticle” (MMNP) configuration on a fiber-optic SPR sensor system in the present work. For this, a gold-coated optical fiber (with an exposed core in the central portion) was used, and this gold-coated surface was first functionalized with the Self-Assembled Monolayer (SAM) of 4-aminothiophenol (4-ATP) molecules. The 4-ATP SAMs were functionalized with biotin molecules through EDC/NHS coupling agents. Separately, citrate-capped gold nanoparticles (~20 nm) were synthesized and subsequently functionalized with streptavidin protein, which exhibits exceptional binding affinity towards biotin molecules. To establish the MMNP configuration, streptavidin-functionalized gold nanoparticles were flown over a biotin-functionalized SPR sensor surface. The Raman signal of 4-ATP molecules in the SPR sensor region was collected before and after interaction with streptavidin-functionalized gold nanoparticles. The Raman signals of the sensor region exhibited huge enhancement after interaction with gold nanoparticles, confirming the creation of the hypothesized MMNP configuration on successful biomolecular interaction between streptavidin-functionalized gold nanoparticles and biotin molecules on the SPR sensor surface. Concomitant with the Raman signal enhancement, a marked shift (~ 3-fold) in the SPR peak is also observed when interacting with the gold nanoparticles.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Aditi Chopra, Girish C. Mohanta, and Sudipta Sarkar Pal "Exploring metal-molecule-metal nanoparticles (MMNP) configuration for introducing specificity in SPR biosensors", Proc. SPIE 13083, SPIE Future Sensing Technologies 2024, 130830P (28 May 2024); https://doi.org/10.1117/12.3021750

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