Real-time vivo imaging by a light sheet microscopy with Airy beam and tilted cylindrical lens

Guoxuan Liu

doi:10.1117/12.3034315

5 July 2024 Real-time vivo imaging by a light sheet microscopy with Airy beam and tilted cylindrical lens

Guoxuan Liu

Proceedings Volume 13183, International Conference on Optoelectronic Information and Functional Materials (OIFM 2024); 131830W (2024) https://doi.org/10.1117/12.3034315
Event: The 3rd International Conference on Optoelectronic Information and Functional Materials (OIFM 2024), 2024, Wuhan, China

Abstract

Nowadays, the medical community lacks a tool on imaging the cerebral hemorrhage process in real time in order to rapidly analyze the dynamic process in cerebral hemorrhage. Against this background, this study proposes a light-sheet fluorescence microscopy imaging technique based on a tilted cylindrical lens. It introduces a difference through the lens to approach the cubic modulation required for Airy's light-sheet microscopy, which can utilize the diffraction-free and self-healing properties of Airy's beam. Meanwhile, it dramatically improves the effective field of view (FOV) on the basis of ensuring axial resolution, and also makes the whole imaging system more compact and less costly. According to the experimental results, this technique can effectively realize the real-time vivo imaging of zebrafish embryonic brain hemorrhage process, and can complete the real-time analysis of the whole process of blood vessel rupture, and collect the real-time information of the brain hemorrhage process with high temporal resolution. This study can focus on the dynamic process of cerebral hemorrhage, which is more advantageous than the traditional method that can only analyze the results. Moreover, this study can realize rapid 3D visualization and analysis of imaging data without processing the original imaging data, which greatly improves the operation efficiency.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Guoxuan Liu "Real-time vivo imaging by a light sheet microscopy with Airy beam and tilted cylindrical lens", Proc. SPIE 13183, International Conference on Optoelectronic Information and Functional Materials (OIFM 2024), 131830W (5 July 2024); https://doi.org/10.1117/12.3034315

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