Fluorescence-lifetime-based sensing of blood gases and cations

Joseph R. Lakowicz; Henryk Szmacinski; Klaus W. Berndt

doi:10.1117/12.58296

16 April 1992 Fluorescence-lifetime-based sensing of blood gases and cations

Joseph R. Lakowicz, Henryk Szmacinski, Klaus W. Berndt

Proceedings Volume 1648, Fiber Optic Medical and Fluorescent Sensors and Applications; (1992) https://doi.org/10.1117/12.58296
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

Chemical sensing with fluorescent probes is usually accomplished by measuring the fluorescence intensity or using wavelength-ratiometric probes. Measurement of fluorescence decay times, rather than intensities, is advantageous because the decay times are largely independent of intensity changes due to light losses, photobleaching, or probe washout. Present technology allows measurement of nanosecond decay times by the phase-modulation method with inexpensive and robust instrumentation. This report describes the use of phase- modulation fluorometry for lifetime-based sensing of O₂, pH, Ca²⁺ and K⁺.

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Joseph R. Lakowicz, Henryk Szmacinski, and Klaus W. Berndt "Fluorescence-lifetime-based sensing of blood gases and cations", Proc. SPIE 1648, Fiber Optic Medical and Fluorescent Sensors and Applications, (16 April 1992); https://doi.org/10.1117/12.58296

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