Probing the reaction coordinate for ligand binding in hemoglobin using ultrafast transient Raman spectroscopy

Huiping Zhu; Robert Lingle Jr.; Xiaobing Xu; John B. Hopkins

doi:10.1117/12.145250

1 May 1993 Probing the reaction coordinate for ligand binding in hemoglobin using ultrafast transient Raman spectroscopy

Huiping Zhu, Robert Lingle Jr., Xiaobing Xu, John B. Hopkins

Author Affiliations +

Proceedings Volume 1890, Biomolecular Spectroscopy III; (1993) https://doi.org/10.1117/12.145250
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Transient picosecond Raman spectroscopy is capable of differentiating vibrational relaxation from conformational changes by comparing the Stokes and anti-Stokes dynamics. We report pump-probe picosecond Raman experiments on oxy- and deoxyhemoglobin (oxyHb and deoxyHb, respectively) using 8 ps 532 nm pump pulses and 8 ps 355 nm probe pulses. Heme- to-protein vibrational cooling has been directly observed in deoxyHb for the first time, and the deconvolved cooling time constant is measured to be 2 - 5 ps. By applying our mode-specific Stokes and anti-Stokes technique to oxyHb, we find that any geminate recombination of photodeligated O₂ must occur in either less than two picoseconds or longer than a nanosecond.

Citation Download Citation

Huiping Zhu, Robert Lingle Jr., Xiaobing Xu, and John B. Hopkins "Probing the reaction coordinate for ligand binding in hemoglobin using ultrafast transient Raman spectroscopy", Proc. SPIE 1890, Biomolecular Spectroscopy III, (1 May 1993); https://doi.org/10.1117/12.145250

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available