Motion estimation and visualization for four-dimensional optical microscopy

Wallace Frank Marshall; David A. Agard; John W. Sedat

doi:10.1117/12.172088

4 April 1994 Motion estimation and visualization for four-dimensional optical microscopy

Wallace Frank Marshall, David A. Agard, John W. Sedat

Proceedings Volume 2184, Three-Dimensional Microscopy: Image Acquisition and Processing; (1994) https://doi.org/10.1117/12.172088
Event: IS&T/SPIE 1994 International Symposium on Electronic Imaging: Science and Technology, 1994, San Jose, CA, United States

Abstract

Four-dimensional (4D) microscopy, the acquisition of time-resolved 3D images of living biological samples, provides not only structural data but also contains information on the mechanics and forces inside a living cell. In order to extract, quantify, and visualize such information for chromosomes, we are exploring simple computational methods that will allow quantitative estimation of chromosome motion within 4D images of living nuclei. A correspondence-based approach is required due to limited temporal resolution. The chromosomes we are tracking are relatively textureless, but additional structural constraints can be imposed to discriminate against false matches.

Citation Download Citation

Wallace Frank Marshall, David A. Agard, and John W. Sedat "Motion estimation and visualization for four-dimensional optical microscopy", Proc. SPIE 2184, Three-Dimensional Microscopy: Image Acquisition and Processing, (4 April 1994); https://doi.org/10.1117/12.172088

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