Changes in the light path length of blood-perfused rat liver by increased hematocrit and anoxia

Toshiyuki Kitai; Bertrand Beauvoit; Hanli Liu; Britton Chance

doi:10.1117/12.200859

31 January 1995 Changes in the light path length of blood-perfused rat liver by increased hematocrit and anoxia

Toshiyuki Kitai, Bertrand Beauvoit, Hanli Liu, Britton Chance

Proceedings Volume 2326, Photon Transport in Highly Scattering Tissue; (1995) https://doi.org/10.1117/12.200859
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

The purpose of this study is to evaluate the usefulness of time-resolved spectroscopy (TRS) and phase modulation spectroscopy (PMS) for the measurement of hemoglobin saturation (SO₂) in the liver. Our materials and methods were: (1) Absorption coefficient ((mu) a) and reduced scattering coefficient (microsecond(s) ') of in situ rat liver, blood-free rat liver and red blood cell (RBC) suspension were measured by TRS. (2) Changes in the light path length of blood-perfused rat liver by increasing hematocrit (3%, 12%, and 36%) and anoxia were measured by PMS. Our results show: (1) (mu) a of in situ rat liver could not be determined, because the absorption was too high. From (mu) a and microsecond(s) ' values of blood-free liver and RBC suspension, (mu) a and microsecond(s) ' of normal-hematocrit liver was extrapolated as 1.08 cm^-1 and 15.46 cm^-1 at 780 nm, respectively. (2) Although the trend of liver SO₂ by increasing hematocrit was reasonable, there was a discrepancy between liver SO₂ and output SO₂ by anoxia. Changes in the light path length of the liver were as small as 10% of the total light path length. We conclude: Quantitation of liver SO₂ by TRS and PMS was difficult because of its very high (mu) a. Since changes in the light path length were small, continuous wave spectroscopy would be an effective way to monitor the oxygenation state of the liver.

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Toshiyuki Kitai, Bertrand Beauvoit, Hanli Liu, and Britton Chance "Changes in the light path length of blood-perfused rat liver by increased hematocrit and anoxia", Proc. SPIE 2326, Photon Transport in Highly Scattering Tissue, (31 January 1995); https://doi.org/10.1117/12.200859

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KEYWORDS

Liver

Phase modulation

Blood

Absorption

Tissues

Spectroscopy

Time resolved spectroscopy

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