Contractile properties of myofibrillar bundles and fusion of sarcolemma vesicles of normal and diseased skeletal muscle using UV-laser microbeam dissection and IR-optical trapping

Claudia Veigel; Dorothea von Pfeil; Abdellah Harim; Rainer H.A. Fink

doi:10.1117/12.197516

22 December 1994 Contractile properties of myofibrillar bundles and fusion of sarcolemma vesicles of normal and diseased skeletal muscle using UV-laser microbeam dissection and IR-optical trapping

Claudia Veigel, Dorothea von Pfeil, Abdellah Harim, Rainer H.A. Fink

Author Affiliations +

Proceedings Volume 2328, Biomedical Optoelectronic Devices and Systems II; (1994) https://doi.org/10.1117/12.197516
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

Applying ultraviolet-laser microdissection enabled us to obtain very small myofibrillar preparations (e.g. 3 micrometers diameter) of normal and diseased muscle fibers which develop forces in the range of nano to micronewton too small to be measured with conventional force transducers. For the present study we built a very sensitive force transducer based on determining the force induced light beam deflection of a 50 micrometers optical fiber, connected to a 635 nm diode laser and detected by two photodiodes at two oppositely positioned 100 micrometers optical fibers to measure forces of smallest myofibrillar preparations. Also, for electrophysiological and stability studies selected vesicles of normal and myopathic fibers were trapped by the infrared-microbeam, brought into close membrane contact with other vesicles and fused by single pulses of the ultraviolet-microbeam. This approach allows for the first time to study membrane properties of normal and myopathic tissue in one preparation under the same intra and extravesicular medium conditions. These 'hybrid' vesicles should be of particular importance for studies of structural stability and electrophysiological properties or lateral mobility of ion channels, e.g. in presence or absence of the membrane-bound cytoskeleton dystrophin-glycoprotein complex which is less or not at all expressed in mdx-mice and Duchenne/Becker patients.

Citation Download Citation

Claudia Veigel, Dorothea von Pfeil, Abdellah Harim, and Rainer H.A. Fink "Contractile properties of myofibrillar bundles and fusion of sarcolemma vesicles of normal and diseased skeletal muscle using UV-laser microbeam dissection and IR-optical trapping", Proc. SPIE 2328, Biomedical Optoelectronic Devices and Systems II, (22 December 1994); https://doi.org/10.1117/12.197516

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
12 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Transducers

Optical fibers

Ultraviolet radiation

Waveguides

Microscopes

Photodiodes

Ion channels

Show All Keywords

Keywords/Phrases

Search In:

Publication Years