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1 March 1995 Phototransformation of ALA-induced protoporphyrin IX (PPIX) in carcinoma cells and of exogenous PPIX in cells and solutions
W. Dietel, Ronald Wendenburg
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Proceedings Volume 2371, 5th International Photodynamic Association Biennial Meeting; (1995) https://doi.org/10.1117/12.203418
Event: Fifth International Photodynamic Association Biennial Meeting, 1994, Amelia Island, FL, United States
Abstract
The fluorescence bleaching and photoproduct formation of PPIX was investigated in Ehrlich ascite carcinoma (EAC) cells (ALA induced and exogenous applied) as well as in micelles (Triton X-100) and DMSO. In all the cases fluorescence bleaching of PPIX occurs but the photoproduct formation is quite different. Besides the rapid fluorescence bleaching of the ALA induced PPIX in EAC cells the formation of a water soluble porphyrin with fluorescence maxima at 616 nm and 678 nm (coproporphyrin) was observed. In the presence of the triplet- and oxygen-quencher (beta) -carotene the fluorescence bleaching of PPIX can be reduced.
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W. Dietel and Ronald Wendenburg "Phototransformation of ALA-induced protoporphyrin IX (PPIX) in carcinoma cells and of exogenous PPIX in cells and solutions", Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); https://doi.org/10.1117/12.203418
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Luminescence
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