Imaging microtubules in buffer solution using tapping mode atomic force microscopy

Monika Fritz; Manfred Radmacher; Miriam W. Allersma; Jason P. Cleveland; Russell J. Stewart; Paul K. Hansma; Christoph F. Schmidt

doi:10.1117/12.205923

30 March 1995 Imaging microtubules in buffer solution using tapping mode atomic force microscopy

Monika Fritz, Manfred Radmacher, Miriam W. Allersma, Jason P. Cleveland, Russell J. Stewart, Paul K. Hansma, Christoph F. Schmidt

Author Affiliations +

Proceedings Volume 2384, Scanning Probe Microscopies III; (1995) https://doi.org/10.1117/12.205923
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Taxol stabilized microtubules were imaged in their native state in buffer solution without any fixation by an atomic force microscope (AFM) operated in tapping mode in liquids. Glass cover slips were derivatized with a positively charged silane to adsorb the filaments. The adsorbed microtubules could be imaged stably without any visible damage for hours. In cases where a microtubule crossed above another one, it often broke to follow the curvature of the underlying one. Longitudinal structures with a spacing of about 8 nm could be resolved suggesting they are protofilaments.

Citation Download Citation

Monika Fritz, Manfred Radmacher, Miriam W. Allersma, Jason P. Cleveland, Russell J. Stewart, Paul K. Hansma, and Christoph F. Schmidt "Imaging microtubules in buffer solution using tapping mode atomic force microscopy", Proc. SPIE 2384, Scanning Probe Microscopies III, (30 March 1995); https://doi.org/10.1117/12.205923

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