Spectroscopic properties of tryptophan and bacteria

Gui Chen Tang; Yuanlong Yang; Zuo-Zhu Huang; W. Hua; F. Zhou; S. Cosloy; Robert R. Alfano

doi:10.1117/12.206817

12 April 1995 Spectroscopic properties of tryptophan and bacteria

Gui Chen Tang, Yuanlong Yang, Zuo-Zhu Huang, W. Hua, F. Zhou, S. Cosloy, Robert R. Alfano

Proceedings Volume 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II; (1995) https://doi.org/10.1117/12.206817
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Fluorescence spectra of tryptophan and bacteria were measured at different concentrations using a Mediscience CD-Scan unit. The emission spectra of tryptophan were obtained using an excitation wavelength at 280 nm. The excitation spectra were obtained at the emission of 340 nm. The minimum detectable concentration of tryptophan was found to be 10^-8 M. The emission spectra for bacteria were probed at 340 nm. The minimum detectable number of bacteria in a beam of the excitation light was determined to be about 30. Assuming that the emission band at 340 nm of bacteria comes from tryptophan, the number of tryptophan per bacterium was estimated to be 10⁸. This approach to determine the number is almost consistent with that obtained using a weight method.

Citation Download Citation

Gui Chen Tang, Yuanlong Yang, Zuo-Zhu Huang, W. Hua, F. Zhou, S. Cosloy, and Robert R. Alfano "Spectroscopic properties of tryptophan and bacteria", Proc. SPIE 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II, (12 April 1995); https://doi.org/10.1117/12.206817

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