Development of a penicillin biosensor using a single optical imaging fiber

Brian G. Healey; David R. Walt

doi:10.1117/12.208518

8 May 1995 Development of a penicillin biosensor using a single optical imaging fiber

Brian G. Healey, David R. Walt

Proceedings Volume 2388, Advances in Fluorescence Sensing Technology II; (1995) https://doi.org/10.1117/12.208518
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

A penicillin biosensor has been fabricated by photodepositing penicillin-sensitive polymer matrices and pH-sensitive polymer matrices on different regions of an optical imaging fiber. Penicillin is detected by coupling the enzymatic activity of penicillinase with the pH sensitivity of fluorescein. Penicillin concentration is correlated to the pH change in the microenvironment of the penicillin-sensitive matrix relative to the pH of the sample solution. This dual sensor removes the need to maintain a constant solution pH when measuring penicillin and should enhance greatly the application of biosensors.

Citation Download Citation

Brian G. Healey and David R. Walt "Development of a penicillin biosensor using a single optical imaging fiber", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); https://doi.org/10.1117/12.208518

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