Assessment of different excitation wavelengths for photodetecting neoplastic urothelial lesions by laser-induced autofluorescence spectroscopy

Maurice Anidjar M.D.; Oliver Cussenot; Sigrid Avrillier; Dominique Ettori; Pierre Teillac; Alain Le Duc

doi:10.1117/12.237594

5 April 1996 Assessment of different excitation wavelengths for photodetecting neoplastic urothelial lesions by laser-induced autofluorescence spectroscopy

Maurice Anidjar M.D., Oliver Cussenot, Sigrid Avrillier, Dominique Ettori, Pierre Teillac, Alain Le Duc

Author Affiliations +

Proceedings Volume 2679, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy; (1996) https://doi.org/10.1117/12.237594
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

We have designed a program using laser induced autofluorescence spectroscopy as a possible way to characterize urothelial tumors of the bladder. The autofluorescence spectra were compared between normal, suspicious and tumor areas of human bladder. Three different pulsed laser wavelengths were used for excitation: 308 nm (excimer), 337 nm (nitrogen) and 480 nm (dye laser). Excitation light was delivered by a specially devised multifiber catheter introduced through the working channel of a regular cystoscope under saline irrigation. The fluorescence light was focused into an optical multichannel analyzer detection system. The data was evaluated in 25 patients immediately before resection of a bladder tumor. Spectroscopic results were compared with histopathology. Upon 337 nm and 480 nm excitations, the overall intensity of the fluorescence spectra from bladder tumors was clearly reduced in comparison with normal urothelium, regardless of the stage and the grade of the tumor. upon 308 nm excitation, the shape of tumor fluorescence spectra, including carcinoma in situ, differed drastically from that of normal tissue. In this case, no absolute intensity measurements are needed and clear diagnosis can be achieved from fluorescence intensity ratio (360/440 nm). This spectroscopic study could be particularly useful for the design of a simplified autofluorescence imaging device for real-time routine detection of occult urothelial neoplastic lesions.

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Maurice Anidjar M.D., Oliver Cussenot, Sigrid Avrillier, Dominique Ettori, Pierre Teillac, and Alain Le Duc "Assessment of different excitation wavelengths for photodetecting neoplastic urothelial lesions by laser-induced autofluorescence spectroscopy", Proc. SPIE 2679, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy, (5 April 1996); https://doi.org/10.1117/12.237594

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KEYWORDS

Bladder

Tumors

Luminescence

Tissues

Laser spectroscopy

Visible radiation

Spectroscopy

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