Fluorescence photodetection of head and neck cancer following topical or systemic application of 5-aminolevulinic acid

Andreas Leunig; Kai Rick; Herbert G. Stepp; Ralph Gutmann; Alwin E. Goetz; Reinhold Baumgartner; Jens Feyh

doi:10.1117/12.260713

3 December 1996 Fluorescence photodetection of head and neck cancer following topical or systemic application of 5-aminolevulinic acid

Andreas Leunig, Kai Rick, Herbert G. Stepp, Ralph Gutmann, Alwin E. Goetz, Reinhold Baumgartner, Jens Feyh

Author Affiliations +

Proceedings Volume 2922, Laser Applications in Medicine and Dentistry; (1996) https://doi.org/10.1117/12.260713
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

The aim of photodynamic diagnosis is the complete visualization of all neoplastic lesions in a tumorous organ after topical or systemic application of a tumor selective photosensitizer. In this investigation we performed quantitative fluorescence measurements following topical and systemic application of 5-aminolevulinic acid to head and neck tumors. We investigated 15 patients with neoplastic lesions of the oral cavity and 5 patients with carcinoma of the larynx after rinsing a 0.4 percent-5-ALA solution or inhalation 5 percent-5-ALA. One patient was given 5-ALA systemically p.o. in a concentration of 10mg/kg b.w. Time course and type of porphyrin accumulation were analyzed in neoplastic and surrounding normal tissue by measuring emission spectra of ALA-induced protoporphyrin IX fluorescence at regular intervals for up to 3 hours following 15 minutes of continuous rinsing of a 0.4 percent- ALA-solution, 1 hour of continuous inhalation and 3 hours after p.o. application. After excitation with violet light of a high pressure xenon arc lamp, fluorescence images in the red spectral range from the tumor tissue and the corresponding macroscopic visible tumor were recorded with a CCD-camera. A quantitative analysis of the fluorescence contrast in neoplastic and surrounding tissue was performed using an optical multichannel analyzer.

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Andreas Leunig, Kai Rick, Herbert G. Stepp, Ralph Gutmann, Alwin E. Goetz, Reinhold Baumgartner, and Jens Feyh "Fluorescence photodetection of head and neck cancer following topical or systemic application of 5-aminolevulinic acid", Proc. SPIE 2922, Laser Applications in Medicine and Dentistry, (3 December 1996); https://doi.org/10.1117/12.260713

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KEYWORDS

Luminescence

Tumors

Tissues

Cancer

Tissue optics

Head

Neck

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