In-situ localization of a photosensitizer as evaluated by vital microscopy

Pal Kristian Selbo; Tor Brustad; Torhild Voss; Johan Moan; Kristian Berg

doi:10.1117/12.297802

29 December 1997 In-situ localization of a photosensitizer as evaluated by vital microscopy

Pal Kristian Selbo, Tor Brustad, Torhild Voss, Johan Moan, Kristian Berg

Proceedings Volume 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III; (1997) https://doi.org/10.1117/12.297802
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

The present study is based on the use of vital microscopy and a charge couple device camera for localization of a porphyrin in normal and neoplastic tissue in nude athymic Balb/c nu/nu mice. Granular tissue was allowed to grow into the space between two optical widows, separated by a 40 micrometer thick spacer in a transparent chamber. WiDr cells derived from a primary adenocarcinoma of the rectosigmoid colon, were used as a tumor model. Tumor cells were grown in vitro as spheroids and transplanted to the tissue in the chamber. The photosensitizer, meso(tetra-phenyl)porphine with two sulfonate groups on adjacent phenylrings (TPPS_2a), was injected i.p. to a concentration of 25 mg kg^-1, and the porphyrin localization was evaluated in vivo and in situ by a fluorescence microscopical technique. A significant difference between normal and neoplastic tissue was observed, with a much faster uptake of the photosensitizer in tumor tissue than in normal tissue.

Citation Download Citation

Pal Kristian Selbo, Tor Brustad, Torhild Voss, Johan Moan, and Kristian Berg "In-situ localization of a photosensitizer as evaluated by vital microscopy", Proc. SPIE 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III, (29 December 1997); https://doi.org/10.1117/12.297802

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