Kinetics of the accumulation of aluminum(III)-sulfophthalocyanine by human leukocytes measured with a scanning flow cytometer

I. G. Scribunov; Pit A. Tarasov; K. A. Semianov; Valerii Pavlovich Maltsev; A. V. Chernyshev; E. R. Chernych; M. V. Tichonova; C. D. Nikonov

doi:10.1117/12.405930

3 November 2000 Kinetics of the accumulation of aluminum(III)-sulfophthalocyanine by human leukocytes measured with a scanning flow cytometer

I. G. Scribunov, Pit A. Tarasov, K. A. Semianov, Valerii Pavlovich Maltsev, A. V. Chernyshev, E. R. Chernych, M. V. Tichonova, C. D. Nikonov

Author Affiliations +

Proceedings Volume 4162, Controlling Tissue Optical Properties: Applications in Clinical Study; (2000) https://doi.org/10.1117/12.405930
Event: EOS/SPIE European Biomedical Optics Week, 2000, Amsterdam, Netherlands

Abstract

The kinetics of aluminum (III)-sulfophthalocyanine uptake by human leukocytes was measured with a scanning flow cytometer (SFC) during the initial period of accumulation, 40 min. The individual cells were distinguished by SFC from their light scattering traces. The dye fluorescence in the cells was excited by N2 pulse laser, and the kinetics of the cell distribution on the amount of the accumulated dye was obtained. A mathematical model of endocytosis was applied in order to describe the dynamics of cell distribution in the system during the cellular uptake. The main kinetic parameters of the dye accumulation were evaluated.

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I. G. Scribunov, Pit A. Tarasov, K. A. Semianov, Valerii Pavlovich Maltsev, A. V. Chernyshev, E. R. Chernych, M. V. Tichonova, and C. D. Nikonov "Kinetics of the accumulation of aluminum(III)-sulfophthalocyanine by human leukocytes measured with a scanning flow cytometer", Proc. SPIE 4162, Controlling Tissue Optical Properties: Applications in Clinical Study, (3 November 2000); https://doi.org/10.1117/12.405930

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