Optical investigation of functional structures in isolated perfused pig heart

Robert Rauh M.D.; Markus Boehnert; Christine Mahlke; Manfred D. Kessler

doi:10.1117/12.405944

3 November 2000 Optical investigation of functional structures in isolated perfused pig heart

Robert Rauh M.D., Markus Boehnert, Christine Mahlke, Manfred D. Kessler

Proceedings Volume 4162, Controlling Tissue Optical Properties: Applications in Clinical Study; (2000) https://doi.org/10.1117/12.405944
Event: EOS/SPIE European Biomedical Optics Week, 2000, Amsterdam, Netherlands

Abstract

Light scattering in tissue of mammals and humans is affected by subcellular structures. Since these structures correlate well with the status of cells and tissue, light scattering seems to be ideal for monitoring of functional tissue state. By use of EMPHO SSK Oxyscan we investigated functional parameters in a novel kind of isolated perfused pig heart model. In this perfusion model we use organs obtained by the local slaughterhouse that are reanimated at our institute by application of a heart-lung machine. By creating 3D-images of tissue scattering we found an interesting relation between anatomical structures of myocardium and the 3D-images. Additionally, we detected coherence between backscattered light intensity and functional tissue status. Furthermore, we got a sight into the redox state of cytochrome aa₃, b and c by creating difference spectra. We believe that this new kind of tissue imaging method will give us the opportunity to get new insights into myocardial function.

Citation Download Citation

Robert Rauh M.D., Markus Boehnert, Christine Mahlke, and Manfred D. Kessler "Optical investigation of functional structures in isolated perfused pig heart", Proc. SPIE 4162, Controlling Tissue Optical Properties: Applications in Clinical Study, (3 November 2000); https://doi.org/10.1117/12.405944

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