High-resolution imaging of biological samples

Ulrike Tauer; Reiner Rygiel; Martin Hoppe

doi:10.1117/12.478009

9 July 2003 High-resolution imaging of biological samples

Ulrike Tauer, Reiner Rygiel, Martin Hoppe

Proceedings Volume 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X; (2003) https://doi.org/10.1117/12.478009
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Confocal laser scanning microscopy is used in many different fields of research nowadays. Therefore, high spatial resolution is required as well as high temporal resolution. Further, the quality of the resulting images has to be considered. Doing imaging with laser scanning microscopes, the balance between spatial resolution, speed and signal-to-noise ratio has to be defined for every specimen and experiment individually. Special adaptations to standard laser scanning microscopes improve the efficiency of the Leica TCS SP2 and Leica TCS SP2 RS for high-resolution low-noise imaging. Here, we want to report from the acousto-optical beam splitter, the resonant K-scanner and new objectives.

Citation Download Citation

Ulrike Tauer, Reiner Rygiel, and Martin Hoppe "High-resolution imaging of biological samples", Proc. SPIE 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X, (9 July 2003); https://doi.org/10.1117/12.478009

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