Imaging protein-protein interactions by multiphoton FLIM

Simon M. Ameer-Beg; Natasha Edme; Marion Peter; Paul R. Barber; Tony Ng; Borivoj Vojnovic

doi:10.1117/12.500544

9 October 2003 Imaging protein-protein interactions by multiphoton FLIM

Simon M. Ameer-Beg, Natasha Edme, Marion Peter, Paul R. Barber, Tony Ng, Borivoj Vojnovic

Proceedings Volume 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging; (2003) https://doi.org/10.1117/12.500544
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

We demonstrate the applicability of time-correlated single photon counting multiphoton microscopy to the spatio-temporal localisation of protein-protein interactions in situ. Examples of new fluorescent protein variants with enhanced properties are given and the development of FRET biosensors for simultaneous measurement of multiple intra- and inter-molecular interactions is illustrated by experimental evidence of an energy transfer cascade via multiple acceptors. The juxtaposition of interacting population and FRET efficiency is elucidated, with a priori knowledge, by multi-exponential analysis.

Citation Download Citation

Simon M. Ameer-Beg, Natasha Edme, Marion Peter, Paul R. Barber, Tony Ng, and Borivoj Vojnovic "Imaging protein-protein interactions by multiphoton FLIM", Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); https://doi.org/10.1117/12.500544

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