Photometrical and dosimetrical measurements for photodynamic diagnosis and therapy with ALA and h-ALA induced PpIX

Dirk Huettenberger; Hans-Jochen Foth

doi:10.1117/12.501185

16 October 2003 Photometrical and dosimetrical measurements for photodynamic diagnosis and therapy with ALA and h-ALA induced PpIX

Dirk Huettenberger, Hans-Jochen Foth

Author Affiliations +

Proceedings Volume 5142, Therapeutic Laser Applications and Laser-Tissue Interactions; (2003) https://doi.org/10.1117/12.501185
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

The quantification of the fluorescence is important to design optical equipment for Photodynamic Diagnosis. The fluorescence intensity of Protoporphyrin IX in solution and induced in cells, cultivated in the hen's eggs model, have been measured photometrically. With an optimized CCD-camera-systems fluorescent tumor areas were detected before and after PDT. The ratio of dead cells was detected by staining with trypan blue after PDT. The measurements were carried out various energy densities at the time of maximal PpIX-enrichement.

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Dirk Huettenberger and Hans-Jochen Foth "Photometrical and dosimetrical measurements for photodynamic diagnosis and therapy with ALA and h-ALA induced PpIX", Proc. SPIE 5142, Therapeutic Laser Applications and Laser-Tissue Interactions, (16 October 2003); https://doi.org/10.1117/12.501185

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