Protoporphyrin IX distribution in rat brain following administration of 5-aminolevulinic acid or its hexylester

Steen J. Madsen; Scott A. Friesen; Geir Olav Hjortland; Olav Engebraaten; Qian Peng; Henry Hirschberg

doi:10.1117/12.527791

13 July 2004 Protoporphyrin IX distribution in rat brain following administration of 5-aminolevulinic acid or its hexylester

Steen J. Madsen, Scott A. Friesen, Geir Olav Hjortland, Olav Engebraaten, Qian Peng, Henry Hirschberg

Author Affiliations +

Proceedings Volume 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV; (2004) https://doi.org/10.1117/12.527791
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

The distribution of protoporphyrin IX (Pp IX) was investigated in a novel animal model followng administration of 5-aminolevulinic acid (ALA) or its hexyl ester (h-ALA). Pre-cultured tumor spheroids prepared from cell lines or tumor biopsy fragments were injected into the brains of immunodeficient rats. Approximately 3 months after spheroid implantation, Rowett nude rats were injected intra-cranially with the maximum tolerable ALA or h-ALA dose. Animals were sacrificed 4 hours post-injection and their brains removed for fluorescence microscopy analysis. The primary finding of this study is that, in the tumor biopsy model, the tumor-to-normal brain PpIX fluorescence ratio is approximately 3 times higher following direct in situ h-ALA adminstration compared to ALA.

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Steen J. Madsen, Scott A. Friesen, Geir Olav Hjortland, Olav Engebraaten, Qian Peng, and Henry Hirschberg "Protoporphyrin IX distribution in rat brain following administration of 5-aminolevulinic acid or its hexylester", Proc. SPIE 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV, (13 July 2004); https://doi.org/10.1117/12.527791

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