Use of adaptive optics for improved multiphoton imaging

John M. Girkin; Paul N. Marsh

doi:10.1117/12.525659

21 June 2004 Use of adaptive optics for improved multiphoton imaging

John M. Girkin, Paul N. Marsh

Proceedings Volume 5323, Multiphoton Microscopy in the Biomedical Sciences IV; (2004) https://doi.org/10.1117/12.525659
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

We report on the integration of active optical elements in a multiphoton microscope to improve the resolution and overall image quality when imaging deeply into biological samples. Optical models were generated of sample systems and these have been compared with the performance of the complete imaging system. The active elements used were commercially available flexible membrane mirrors controlled by custom, home written, software. Significant improvements in image quality have been demonstrated using a range of optimisation routines based on the analysis of the images produced by the system, rather than with a wavefront sensor. A three-fold increase in the resolution 100μm into the sample was achieved.

Citation Download Citation

John M. Girkin and Paul N. Marsh "Use of adaptive optics for improved multiphoton imaging", Proc. SPIE 5323, Multiphoton Microscopy in the Biomedical Sciences IV, (21 June 2004); https://doi.org/10.1117/12.525659

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