Biosensor-controlled gene therapy/drug delivery with nanoparticles for nanomedicine

Tarl W. Prow; William A. Rose; Nan Wang; Lisa M. Reece; Yuri Lvov; James F. Leary

doi:10.1117/12.589422

1 April 2005 Biosensor-controlled gene therapy/drug delivery with nanoparticles for nanomedicine

Tarl W. Prow, William A. Rose, Nan Wang, Lisa M. Reece, Yuri Lvov, James F. Leary

Proceedings Volume 5692, Advanced Biomedical and Clinical Diagnostic Systems III; (2005) https://doi.org/10.1117/12.589422
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Nanomedicine involves cell-by-cell regenerative medicine, either repairing cells one at a time or triggering apoptotic pathways in cells that are not repairable. Multilayered nanoparticle systems are being constructed for the targeted delivery of gene therapy to single cells. Cleavable shells containing targeting, biosensing, and gene therapeutic molecules are being constructed to direct nanoparticles to desired intracellular targets. Therapeutic gene sequences are controlled by biosensor-activated control switches to provide the proper amount of gene therapy on a single cell basis. The central idea is to set up gene therapy "nanofactories" inside single living cells. Molecular biosensors linked to these genes control their expression. Gene delivery is started in response to a biosensor detected problem; gene delivery is halted when the cell response indicates that more gene therapy is not needed. Cell targeting of nanoparticles, both nanocrystals and nanocapsules, has been tested by a combination of fluorescent tracking dyes, fluorescence microscopy and flow cytometry. Intracellular targeting has been tested by confocal microscopy. Successful gene delivery has been visualized by use of GFP reporter sequences. DNA tethering techniques were used to increase the level of expression of these genes. Integrated nanomedical systems are being designed, constructed, and tested in-vitro, ex-vivo, and in small animals. While still in its infancy, nanomedicine represents a paradigm shift in thinking-from destruction of injured cells by surgery, radiation, chemotherapy to cell-by-cell repair within an organ and destruction of non-repairable cells by natural apoptosis.

Citation Download Citation

Tarl W. Prow, William A. Rose, Nan Wang, Lisa M. Reece, Yuri Lvov, and James F. Leary "Biosensor-controlled gene therapy/drug delivery with nanoparticles for nanomedicine", Proc. SPIE 5692, Advanced Biomedical and Clinical Diagnostic Systems III, (1 April 2005); https://doi.org/10.1117/12.589422

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available