Adaptation of nanoarrays for the study of alpha-synuclein aggregation: preliminary results

Heidelinde Regina Christina Dietrich; Richard L. van den Doel; Wolfgang Hoyer; Wim van Oel; Guus Liqui Lung; Yuval Garini; Thomas Jovin; Ian T. Young

doi:10.1117/12.587493

29 March 2005 Adaptation of nanoarrays for the study of α-synuclein aggregation: preliminary results

Heidelinde Regina Christina Dietrich, Richard L. van den Doel, Wolfgang Hoyer, Wim van Oel, Guus Liqui Lung, Yuval Garini, Thomas Jovin, Ian T. Young

Author Affiliations +

Proceedings Volume 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III; (2005) https://doi.org/10.1117/12.587493
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

In previous publications we have shown that we can perform enzymatic reactions in nanoarrays by means of a microarray-reader based on a conventional microscope. In this publication we report on a modification of this system in order to monitor the aggregation kinetics of the natively unfolded protein α-synuclein. We describe the motivation for this development, the problems associated with the miniaturization of the aggregation assay, and the validation of our modifications.

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Heidelinde Regina Christina Dietrich, Richard L. van den Doel, Wolfgang Hoyer, Wim van Oel, Guus Liqui Lung, Yuval Garini, Thomas Jovin, and Ian T. Young "Adaptation of nanoarrays for the study of α-synuclein aggregation: preliminary results", Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); https://doi.org/10.1117/12.587493

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