Chemical and biological sensing through optical resonances in microcavities

Melikhan Tanyeri; Mikaela Nichkova; Bruce D Hammock; Ian M. Kennedy

doi:10.1117/12.588169

29 March 2005 Chemical and biological sensing through optical resonances in microcavities

Melikhan Tanyeri, Mikaela Nichkova, Bruce D Hammock, Ian M. Kennedy

Proceedings Volume 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III; (2005) https://doi.org/10.1117/12.588169
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

A microdroplet or a latex microsphere often acts as an optical cavity that supports Morphology Dependent Resonances (MDRs) at wavelengths where the droplet circumference is an integer multiple of the emission wavelength. Enhanced radiative energy transfer through these optical resonances can also be utilized as a transduction mechanism for chemical and biological sensing. Enhancement in radiative energy transfer is observed when a donor/acceptor pair is present in the resonant medium of a microcavity. Here, we demonstrate avidin-biotin binding and its detection through a FRET pair as a potential application for ultra-sensitive detection for fluoroimmunoassays. The binding interaction between the biotinylated fluorescent beads (donor) and streptavidin-Alexa Fluor 555 (acceptor) conjugate was used to observe the energy transfer between the dye pairs. Strong coupling of acceptor emission into optical resonances shows that the energy transfer is efficiently mediated through these resonances.

Citation Download Citation

Melikhan Tanyeri, Mikaela Nichkova, Bruce D Hammock, and Ian M. Kennedy "Chemical and biological sensing through optical resonances in microcavities", Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); https://doi.org/10.1117/12.588169

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