Three dimensional confocal microscopy of fluorescent microspheres: imaging and size determination

Y. P. Rakovich D.V.M.; L. Yang; C. M. Taylor; L. A. Dunbar; Aaron Mac Raighne; J. F. Donegan; Eithne M. McCabe

doi:10.1117/12.604380

8 June 2005 Three dimensional confocal microscopy of fluorescent microspheres: imaging and size determination

Y. P. Rakovich D.V.M., L. Yang, C. M. Taylor, L. A. Dunbar, Aaron Mac Raighne, J. F. Donegan, Eithne M. McCabe

Proceedings Volume 5827, Opto-Ireland 2005: Photonic Engineering; (2005) https://doi.org/10.1117/12.604380
Event: OPTO-Ireland, 2005, Dublin, Ireland

Abstract

In this paper, we experimentally studied both the bright-field and fluorescence images of microspheres by conventional and confocal scanning polarization microscopes. A qualitative analysis have been given to show a physical picture on the imaging of the microspheres. Emission spectra from melamine formaldehyde microspheres stained with Ethidium Bromide or covered by thin shell of CdTe nanocrystals have been experimentally studied. We adopted analytical expressions describing the resonance spacing in order to determine the size of the microspheres.

Citation Download Citation

Y. P. Rakovich D.V.M., L. Yang, C. M. Taylor, L. A. Dunbar, Aaron Mac Raighne, J. F. Donegan, and Eithne M. McCabe "Three dimensional confocal microscopy of fluorescent microspheres: imaging and size determination", Proc. SPIE 5827, Opto-Ireland 2005: Photonic Engineering, (8 June 2005); https://doi.org/10.1117/12.604380

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