Imaging the bone marrow stem cells morphogenesis in PGA scaffold by multiphoton autofluorescence and second harmonic (SHG) imaging

Hsuan-Shu Lee; Shu-Wen Teng; Hsiao-Ching Chen; Wen Lo; Yen Sun; Tze-Yu Lin; Ling-Ling Chiou; Ching-Chuan Jiang; Chen-Yuan Dong

doi:10.1117/12.647607

1 March 2006 Imaging the bone marrow stem cells morphogenesis in PGA scaffold by multiphoton autofluorescence and second harmonic (SHG) imaging

Hsuan-Shu Lee, Shu-Wen Teng, Hsiao-Ching Chen, Wen Lo, Yen Sun, Tze-Yu Lin, Ling-Ling Chiou, Ching-Chuan Jiang, Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 6084, Optical Interactions with Tissue and Cells XVII; 60841C (2006) https://doi.org/10.1117/12.647607
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

The ability to image tissue engineering products without damaging histological procedures is important for the understanding of the dynamics of tissue reorganization and formation. In this work, we test the ability of multiphoton autofluorescence and second harmonic generation microscopy to image engineered tissues following chrondrogenic induction. The system we used is human bone marrow stem cells seeded in the scaffold polyglycolic acid (PGA). Our results show that autofluorescence can be used to image cells while second harmonic generation signal can be used to visualize the synthesis of extracellular matrix. This approach demonstrates the ability of multiphoton imaging in the study of tissue engineering products.

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Hsuan-Shu Lee, Shu-Wen Teng, Hsiao-Ching Chen, Wen Lo, Yen Sun, Tze-Yu Lin, Ling-Ling Chiou, Ching-Chuan Jiang, and Chen-Yuan Dong "Imaging the bone marrow stem cells morphogenesis in PGA scaffold by multiphoton autofluorescence and second harmonic (SHG) imaging", Proc. SPIE 6084, Optical Interactions with Tissue and Cells XVII, 60841C (1 March 2006); https://doi.org/10.1117/12.647607

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