2D multiforce optical tweezers to investigate cell adhesion strengthening in living cells

Valentina Emiliani; Daniele Sanvitto; Christiane Durieux

doi:10.1117/12.661733

20 April 2006 2D multiforce optical tweezers to investigate cell adhesion strengthening in living cells

Valentina Emiliani, Daniele Sanvitto, Christiane Durieux

Proceedings Volume 6195, Nanophotonics; 619508 (2006) https://doi.org/10.1117/12.661733
Event: SPIE Photonics Europe, 2006, Strasbourg, France

Abstract

We present the multi trap optical tweezers system that enables to generate two-dimensional dynamical configurations of focal spot where the trapping force of each element can be individually changed. We apply the system to investigate how substrate rigidity affects the strength of the integrin-extracellular matrix adhesion in living cells. Adhesion sites of different rigidity are mimicked by simultaneously attaching on cell cortex beads held by optical traps of different stiffness. We monitor the effect induced by the local rigidity on cell adhesion by looking at vinculin recruitment in GFP-Vin transfected HeLa cell. To this end the optical tweezers system is inserted in an epifluorescence inverted microscope.

Citation Download Citation

Valentina Emiliani, Daniele Sanvitto, and Christiane Durieux "2D multiforce optical tweezers to investigate cell adhesion strengthening in living cells", Proc. SPIE 6195, Nanophotonics, 619508 (20 April 2006); https://doi.org/10.1117/12.661733

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