The apoptosis induced by HMME-based photodynamic therapy in rabbit vascular smooth muscle cells

Huijuan Yin; Xiaoyuan Li; Hong Lin; Jianzhong Liu; Hongkui Yu

doi:10.1117/12.702042

28 February 2007 The apoptosis induced by HMME-based photodynamic therapy in rabbit vascular smooth muscle cells

Huijuan Yin, Xiaoyuan Li, Hong Lin, Jianzhong Liu, Hongkui Yu

Proceedings Volume 6427, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI; 64271F (2007) https://doi.org/10.1117/12.702042
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Objective To study the effects of HMME-based photodynamic therapy on proliferation and apoptosis of rabbit vascular smooth muscle cells(VSMCs). Method The cytotoxic effect of HMME-PDT on rabbit vascular smooth muscle cells was studied by means of Trypan Blue assay, HMME at 10&mgr;g/ml concentration and the light dose at 2.4~4.8 J/cm² were selected in the studies. The morphological character 24h post-PDT was investigated by HE Staining. Annexin V and propidium iodide (PI) binding assays were performed to analyze the characteristics of cell death after HMME-PDT. Furthermore, The intracellular distributions of the HMME were measured by the confocal laser scanning microscope. Result It was showed the photocytotoxity to VSMC cells was dose related by Trypan Blue assay. Histology observing suggests HMME-PDT could induce cell death through apoptosis or necrosis, and the apoptosic rate was up to 50.5% by AnnexinV /PI assay. Moreover, the fluorescence images of HMME intracellular localization demonstrated that the HMME diffused into the mitochondria. Conclusion HMME-PDT could significantly inhibite VSMC proliferation and induce apoptosis.

Citation Download Citation

Huijuan Yin, Xiaoyuan Li, Hong Lin, Jianzhong Liu, and Hongkui Yu "The apoptosis induced by HMME-based photodynamic therapy in rabbit vascular smooth muscle cells", Proc. SPIE 6427, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI, 64271F (28 February 2007); https://doi.org/10.1117/12.702042

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