Uptake of photosensitizers by bacteria is influenced by the presence of cations

A. Kishen; S. George

doi:10.1117/12.740812

26 April 2007 Uptake of photosensitizers by bacteria is influenced by the presence of cations

A. Kishen, S. George

Proceedings Volume 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII; 65350N (2007) https://doi.org/10.1117/12.740812
Event: Saratov Fall Meeting 2006, 2006, Saratov, Russian Federation

Abstract

This investigation studies the influence of cations on photosensitizer uptake by Enterococcus faecalis (gram positive) and Actinobacillus actinomycetemcomitans (gram negative). Methods- The uptake of Methylene blue (MB) and Indocyanine Green (ICG), by bacteria were studied under the influence of divalent cations (CaCl₂ & MgCl₂) and EDTA. Further, E. faecalis cells subjected to trypsinisation and calcium channel blocker (verapamil) were also analysed for MB and ICG uptake inorder to understand the mechanism of photosensitizer uptake. Results- Uptake of ICG was enhanced in the presence of divalent cations in E. faecalis and A. actinomycetemcomitans. Treating cells with EDTA had no significant effect on the photosensitizer uptake, although the highest concentration tested showed an enhancement of uptake. In contrast to ICG, MB showed a decreased uptake by bacterial cells on subjecting them to divalent cations and EDTA. Calcium channel blocker had no significant inhibitory effect on photosensitizers uptake. However, trypsin treatment resulted in significant reduction of ICG uptake. The result suggested that ICG uptake by bacteria is mediated through specific transporter protein while MB is associated with the outer surface structures of bacterial cells.

Citation Download Citation

A. Kishen and S. George "Uptake of photosensitizers by bacteria is influenced by the presence of cations", Proc. SPIE 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII, 65350N (26 April 2007); https://doi.org/10.1117/12.740812

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