Two- and one-photon color confocal screening microscope

Christian Seebacher; Joachim Walter; Rainer Uhl

doi:10.1117/12.729553

12 July 2007 Two- and one-photon color confocal screening microscope

Christian Seebacher, Joachim Walter, Rainer Uhl

Proceedings Volume 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III; 66300F (2007) https://doi.org/10.1117/12.729553
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

The goal was to develop an upright microscope platform for the screening of slides employing one- and two-photon laser scanning techniques. A highly compact, vibration damping unit was created, which combines novel concepts for moving a slide in three dimensions, keeping it focused while doing so, scanning a laser-focus over the sample using novel galvanometer-control concepts, combining and separating excitation and emission beam and spectrally dispersing the emitted light by a linearized prism-spectrograph. Spectral detection is achieved by turning a 128 x 128 back-thinned EMCCD detector in a continuously reading spectral point-detector. To make the unit even ore versatile, it can be turned into a conventional wide field fluorescence microscope, enabling rapid routine observation to select regions of the sample for a subsequent, more detailed confocal analysis.

Citation Download Citation

Christian Seebacher, Joachim Walter, and Rainer Uhl "Two- and one-photon color confocal screening microscope", Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 66300F (12 July 2007); https://doi.org/10.1117/12.729553

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