Measurement of intracellular pH (pHi) in a single cell using fluorescent probe and fiber optic nanoprobe

Qinmiao Chen; Yishen Qiu; Zhihao Chen; Na Fang; Gaoming Li

doi:10.1117/12.755230

8 January 2008 Measurement of intracellular pH (pH_i) in a single cell using fluorescent probe and fiber optic nanoprobe

Qinmiao Chen, Yishen Qiu, Zhihao Chen, Na Fang, Gaoming Li

Author Affiliations +

Proceedings Volume 6826, Optics in Health Care and Biomedical Optics III; 68262N (2008) https://doi.org/10.1117/12.755230
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

An optical system for measurement of the intracellular pH (pHi) in a single living cell by using the fluorescent probe, 5(6)-carboxyfluorescein diacetate (CFDA) and fiber optic nanoprobe was demonstrated in this work. The CFDA probe is used to determine pHi in the yeast, Saccharomyces cerevisiae 97 while fiber optic nanoprobe is used to guide excitation light and receive emission light within a single cell. Experimental results showed that our system had higher detection sensitivity than other standard spectrometer, which is important to single-cell analysis, especially for the microanalysis in a single-cell.

Citation Download Citation

Qinmiao Chen, Yishen Qiu, Zhihao Chen, Na Fang, and Gaoming Li "Measurement of intracellular pH (pH_i) in a single cell using fluorescent probe and fiber optic nanoprobe", Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 68262N (8 January 2008); https://doi.org/10.1117/12.755230

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available