Imaging stented tissue engineered blood vessel mimics

Garret T. Bonnema; Kristen O. Cardinal; Stuart K. Williams; Jennifer K. Barton

doi:10.1117/12.763991

15 February 2008 Imaging stented tissue engineered blood vessel mimics

Garret T. Bonnema, Kristen O. Cardinal, Stuart K. Williams, Jennifer K. Barton

Proceedings Volume 6858, Optics in Tissue Engineering and Regenerative Medicine II; 68580F (2008) https://doi.org/10.1117/12.763991
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

An ideal vascular stent design promotes a thin anti-thrombogenic cellular lining while avoiding restenosis. To assess the utility of their designs, stent manufactures often use destructive techniques such as scanning electron microscopy to measure the percentage of the stent covered with a cellular lining. In this study, we use a custom-built longitudinal/rotational scanning endoscope and determine the ability of optical coherence tomography (OCT) to quantify the percent cellular coverage of stented tissue engineered blood vessel mimics. Stents were deployed within twelve mimics after 14-days of development in bioreactors. OCT images were acquired within the bioreactor at several time points after the stent deployment. At 20-days post deployment, the mimics were fixed and imaged volumetrically with OCT. Matlab software was developed to automatically calculate the percent cellular coverage from the OCT images. Algorithm results were compared to similar measurements performed with bis-benzimide (BBI) fluorescence imaging and manually calculated percent coverage from three different observers of the OCT images. Progressive accumulation of cellular material on the stents could be visualized with OCT. For the volumetric images, the algorithm calculated percent cellular coverages ranging from 11 to 76%. Good agreement was found between the OCT-based measurements and the other techniques. On average, the algorithm differed less than 5% from the manual percent coverage calculations. OCT together with automated software can provide an accurate, non-destructive measurement of the percent cellular coverage of vascular stents.

Citation Download Citation

Garret T. Bonnema, Kristen O. Cardinal, Stuart K. Williams, and Jennifer K. Barton "Imaging stented tissue engineered blood vessel mimics", Proc. SPIE 6858, Optics in Tissue Engineering and Regenerative Medicine II, 68580F (15 February 2008); https://doi.org/10.1117/12.763991

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Optical coherence tomography

Visualization

Blood vessels

Endoscopes

Arteries

Tissues

Imaging systems

Show All Keywords

Keywords/Phrases

Search In:

Publication Years