Multimodal optical microscopy for monitoring fast neuronal activity and signaling

S. Pagès; I. Veilleux; P. De Koninck; D. Côté

doi:10.1117/12.764161

15 February 2008 Multimodal optical microscopy for monitoring fast neuronal activity and signaling

S. Pagès, I. Veilleux, P. De Koninck, D. Côté

Proceedings Volume 6860, Multiphoton Microscopy in the Biomedical Sciences VIII; 68601X (2008) https://doi.org/10.1117/12.764161
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We present a video-rate optical microscope that allows simultaneous imaging of two-photon excited fluorescence (TPEF), second harmonic generation (SHG) and reflectance. The ms time resolution of the system together with its submicrometer spatial resolution make it an ideal tool for studying fast neuronal activity and signaling, to understand how action potentials are decoded molecularly. Transient trans-membrane potentials are measured with SHG, while the evoked calcium oscillations are monitored with TPEF. The ability of this system to monitor both signals simultaneously in multiple sub-compartments of living neurons should open the way to study how the electrical activity of neurons is encoded intracellularly.

Citation Download Citation

S. Pagès, I. Veilleux, P. De Koninck, and D. Côté "Multimodal optical microscopy for monitoring fast neuronal activity and signaling", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601X (15 February 2008); https://doi.org/10.1117/12.764161

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