Assembly and intracellular delivery of quantum dot-fluorescent protein bioconjugates

Igor L. Medintz; Thomas Pons; James B Delehanty; Kimihiro Susumu; Philip E. Dawson; Hedi Mattoussi

doi:10.1117/12.763702

13 March 2008 Assembly and intracellular delivery of quantum dot-fluorescent protein bioconjugates

Igor L. Medintz, Thomas Pons, James B Delehanty, Kimihiro Susumu, Philip E. Dawson, Hedi Mattoussi

Proceedings Volume 6866, Colloidal Quantum Dots for Biomedical Applications III; 68660B (2008) https://doi.org/10.1117/12.763702
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We have previously assembled semiconductor quantum dot (QD)-based fluorescence resonance energy transfer (FRET) sensors that can specifically detect nutrients, explosives or enzymatic activity. These sensors utilized the inherent benefits of QDs as FRET donors to optimize signal transduction. In this report we functionalize QDs with the multi-subunit multi-chromophore b-phycoerythrin (b-PE) light harvesting complex using biotin-Streptavidin binding. FRET and gel electrophoretic analyses were used to characterize and confirm the QD-b-PE self-assembly. We found that immobilizing additional cell-penetrating peptides on the nanocrystal surface along with the b-PE was the key factor allowing the mixed surface QD-cargos to undergo endocytosis and intracellular delivery. Our findings on the intracellular uptake promoted by CPP were compared to those collected using microinjection technique, where QD-assemblies were delivered directly into the cytoplasm; this strategy allows bypassing of the endocytic uptake pathway. Intracellular delivery of multifunctional QD-fluorescent protein assemblies has potential applications for use in protein tracking, sensing and diagnostics.

Citation Download Citation

Igor L. Medintz, Thomas Pons, James B Delehanty, Kimihiro Susumu, Philip E. Dawson, and Hedi Mattoussi "Assembly and intracellular delivery of quantum dot-fluorescent protein bioconjugates", Proc. SPIE 6866, Colloidal Quantum Dots for Biomedical Applications III, 68660B (13 March 2008); https://doi.org/10.1117/12.763702

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