Low-cost, multiplexed biosensor for disease diagnosis

Christopher J. Myatt; Marie Delaney; Kathryn Todorof; James Heil; Monique Givens; Robert T. Schooley M.D.; Michael J. Lochhead

doi:10.1117/12.811129

19 February 2009 Low-cost, multiplexed biosensor for disease diagnosis

Christopher J. Myatt, Marie Delaney, Kathryn Todorof, James Heil, Monique Givens, Robert T. Schooley M.D., Michael J. Lochhead

Author Affiliations +

Proceedings Volume 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems; 716703 (2009) https://doi.org/10.1117/12.811129
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Cost-effective disease diagnosis in resource-limited settings remains a critical global health challenge. Qualitative rapid tests based on lateral flow technology provide valuable screening information, but require relatively expensive confirmatory tests and generally lack quantitation. We report on a fluorescence technology that combines low cost instrumented readout with passive pumping in a disposable cartridge. The detection system utilizes a novel waveguide illumination approach in conjunction with commercial CMOS imagers. Total instrument cost in production are projected to be around $100 This cost structure and instrument ease of use will enable use in point-of-care settings, outside of centralized laboratories. The system has been used for detection and analysis of proteins, antibodies, nucleic acids, and cells. Here we will report first on our development of a multiplexed, array-based serology assay for HIV and common AIDS co-infections. Data will be presented for HIV/HCV antibody testing in human serum samples. In addition, we will present data on the use of the system for sensitive detection of bacterial RNA. Current detection limit for the model multiplexed RNA sandwich assay is 1 femtomolar target RNA. Finally, a high magnification version of the system is used to image immunostained human T cells.

Citation Download Citation

Christopher J. Myatt, Marie Delaney, Kathryn Todorof, James Heil, Monique Givens, Robert T. Schooley M.D., and Michael J. Lochhead "Low-cost, multiplexed biosensor for disease diagnosis", Proc. SPIE 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems, 716703 (19 February 2009); https://doi.org/10.1117/12.811129

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