Emission spectral analysis of caspase-3 activation during artesunate (ART)-induced apoptosis of human lung adenocarcinoma cell

Wen-liang Pan; Tong-sheng Chen; Junle Qu

doi:10.1117/12.807252

24 February 2009 Emission spectral analysis of caspase-3 activation during artesunate (ART)-induced apoptosis of human lung adenocarcinoma cell

Wen-liang Pan, Tong-sheng Chen, Junle Qu

Author Affiliations +

Proceedings Volume 7178, Biophotonics and Immune Responses IV; 71780I (2009) https://doi.org/10.1117/12.807252
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Artesunate (ART), a semi-synthetic derivative of the sesquiterpene artemisinin extracted from the Chinese herb Artemisia annua, exerts a broad spectrum of clinical activity against human cancers. Artemisinin-derivative combination chemotherapy is recommended by WHO since it acts rapidly and is well tolerated and particularly effective. In present investigation, we used CKK-8 assay to assess the inhibitory effects of ART on human lung adenocarcinoma (ASTC-a-1) cells. Apoptotic activity of ART in ASTC-a-1 cells was detected by means of nuclear staining with Hoechst33258. In order to monitor the activity of caspase-3 during ART-induced ASTC-a-1 cells apoptosis, the dynamical emission spectra of SCAT3, a FRET plasmid based on GFPs, were performed inside living cell expressed stably with SCAT3 after ART treatment. The results showed that (1) ART could inhibit ASTC-a-1 cells proliferation in a dose-dependent manner; (2) chromatin condensation was observed after ART treatment for 48 h; (3) the SCAT3 inside living cells were cleaved after ART treatment for 48 h, implying that caspase-3 was involved in the ART-induced apoptosis.

Citation Download Citation

Wen-liang Pan, Tong-sheng Chen, and Junle Qu "Emission spectral analysis of caspase-3 activation during artesunate (ART)-induced apoptosis of human lung adenocarcinoma cell", Proc. SPIE 7178, Biophotonics and Immune Responses IV, 71780I (24 February 2009); https://doi.org/10.1117/12.807252

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