Comparison of background corrected fluorescence correlation spectroscopy and fluorescence lifetime correlation spectroscopy: dilution series revisited

Steffen Rüttinger; Peter Kapusta; Matthias Patting; Michael Wahl; Rainer Macdonald

doi:10.1117/12.808883

24 February 2009 Comparison of background corrected fluorescence correlation spectroscopy and fluorescence lifetime correlation spectroscopy: dilution series revisited

Steffen Rüttinger, Peter Kapusta, Matthias Patting, Michael Wahl, Rainer Macdonald

Author Affiliations +

Proceedings Volume 7185, Single Molecule Spectroscopy and Imaging II; 718508 (2009) https://doi.org/10.1117/12.808883
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We performed quantitative tests in order to compare the practical limits of FCS and FLCS. Unlike conventional FCS, FLCS yields precise and correct concentration values from as low as picomolar to micromolar concentrations. We discuss some of the inherent technical limitations of FCS and demonstrate that they are easily overcome by FLCS employing the simplest confocal detection scheme.

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Steffen Rüttinger, Peter Kapusta, Matthias Patting, Michael Wahl, and Rainer Macdonald "Comparison of background corrected fluorescence correlation spectroscopy and fluorescence lifetime correlation spectroscopy: dilution series revisited", Proc. SPIE 7185, Single Molecule Spectroscopy and Imaging II, 718508 (24 February 2009); https://doi.org/10.1117/12.808883

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