MicroPET/CT colonoscopy in long-lived Min mouse using NM404

Matthew B. Christensen; Richard B. Halberg; Melissa M. Schutten; Jamey P. Weichert

doi:10.1117/12.811722

27 February 2009 MicroPET/CT colonoscopy in long-lived Min mouse using NM404

Matthew B. Christensen, Richard B. Halberg, Melissa M. Schutten, Jamey P. Weichert

Proceedings Volume 7262, Medical Imaging 2009: Biomedical Applications in Molecular, Structural, and Functional Imaging; 726210 (2009) https://doi.org/10.1117/12.811722
Event: SPIE Medical Imaging, 2009, Lake Buena Vista (Orlando Area), Florida, United States

Abstract

Colon cancer is a leading cause of death in the US, even though many cases are preventable if tumors are detected early. One technique to promote screening is Computed Tomography Colonography (CTC). NM404 is a second generation phospholipid ether analogue which has demonstrated selective uptake and prolonged retention in 43/43 types of malignant tumors but not inflammatory sites or premalignant lesions. The purpose of this experiment was to evaluate (SWR x B6 )F1.Min mice as a preclinical model to test MicroPET/CT dual modality virtual colonoscopy. Each animal was given an IV injection of ¹²⁴I-NM404 (100 uCi) 24, 48 and 96 hours prior to scanning on a dedicated microPET/CT system. Forty million counts were histogrammed in 3D and reconstructed using an OSEM 2D algorithm. Immediately after PET acquisition, a 93 m volumetric CT was acquired at 80 kVp, 800 uA and 350 ms exposures. Following CT, the mouse was sacrificed. The entire intestinal tract was excised, washed, insufflated, and scanned ex vivo A total of eight tissue samples from the small intestine were harvested: 5 were benign adenomas, 2 were malignant adenocarcinomas, and 1 was a Peyer's patch (lymph tissue) . The sites of these samples were positioned on CT and PET images based on morphological cues and the distance from the anus. Only 1/8 samples showed tracer uptake. several hot spots in the microPET image were not chosen for histology. (SWR x B6)F1.Min mice develop benign and malignant tumors, making this animal model a strong candidate for future dual modality microPET/CT virtual colonography studies.

Citation Download Citation

Matthew B. Christensen, Richard B. Halberg, Melissa M. Schutten, and Jamey P. Weichert "MicroPET/CT colonoscopy in long-lived Min mouse using NM404", Proc. SPIE 7262, Medical Imaging 2009: Biomedical Applications in Molecular, Structural, and Functional Imaging, 726210 (27 February 2009); https://doi.org/10.1117/12.811722

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KEYWORDS

Tumors

Intestine

Positron emission tomography

Tissues

In vivo imaging

Computed tomography

Tumor growth modeling

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