Surface plasmon resonance imaging for parallelized detection of protein biomarkers

Marek Piliarik; Lucie Párová; Hana Vaisocherová; Jiří Homola

doi:10.1117/12.820882

18 May 2009 Surface plasmon resonance imaging for parallelized detection of protein biomarkers

Marek Piliarik, Lucie Párová, Hana Vaisocherová, Jiří Homola

Proceedings Volume 7356, Optical Sensors 2009; 73560D (2009) https://doi.org/10.1117/12.820882
Event: SPIE Optics + Optoelectronics, 2009, Prague, Czech Republic

Abstract

We report a novel high-throughput surface plasmon resonance (SPR) biosensor for rapid and parallelized detection of protein biomarkers. The biosensor is based on a high-performance SPR imaging sensor with polarization contrast and internal referencing which yields a considerably higher sensitivity and resolution than conventional SPR imaging systems (refractive index resolution 2 × 10^-7 RIU). We combined the SPR imaging biosensor with microspotting to create an array of antibodies. DNA-directed protein immobilization was utilized for the spatially resolved attachment of antibodies. Using Human Chorionic Gonadotropin (hCG) as model protein biomarker, we demonstrated the potential for simultaneous detection of proteins in up to 100 channels.

Citation Download Citation

Marek Piliarik, Lucie Párová, Hana Vaisocherová, and Jiří Homola "Surface plasmon resonance imaging for parallelized detection of protein biomarkers", Proc. SPIE 7356, Optical Sensors 2009, 73560D (18 May 2009); https://doi.org/10.1117/12.820882

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