Study on the best method of SPIO labeling on the cell line ECV304

FuYuan Yang; MingXi Yu; WenLi Chen; Quan Zhou

doi:10.1117/12.841389

11 February 2010 Study on the best method of SPIO labeling on the cell line ECV304

FuYuan Yang, MingXi Yu, WenLi Chen, Quan Zhou

Proceedings Volume 7565, Biophotonics and Immune Responses V; 75650P (2010) https://doi.org/10.1117/12.841389
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Studies the superparamagnetic iron oxide (SPIO) label ECV304 method and test its effect on cell physiological activity .We use different concentration PLL-SPIO incubate ECV304 cells, Prussian blue dye was taken to examine SPIO mark efficiency, DCF dyeing and PI & Annexin the VFITC double dyeing was carried out to analyze cell apoptosis. The result shows that 25μg/ml gave the most efficient labeling concentration, there is also no toxic effect on cells in this concentration. This outcome can be used in MRI of living body cell transplant tracking.

Citation Download Citation

FuYuan Yang, MingXi Yu, WenLi Chen, and Quan Zhou "Study on the best method of SPIO labeling on the cell line ECV304", Proc. SPIE 7565, Biophotonics and Immune Responses V, 75650P (11 February 2010); https://doi.org/10.1117/12.841389

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