Quantification of colloidal and intracellular gold nanomarkers down to the single particle level using confocal microscopy

Sabine Klein; Svea Petersen; Ulrike Taylor; Detlef Rath; Stephan Barcikowski

doi:10.1117/12.840985

26 February 2010 Quantification of colloidal and intracellular gold nanomarkers down to the single particle level using confocal microscopy

Sabine Klein, Svea Petersen, Ulrike Taylor, Detlef Rath, Stephan Barcikowski

Author Affiliations +

Proceedings Volume 7573, Biomedical Applications of Light Scattering IV; 75730L (2010) https://doi.org/10.1117/12.840985
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

The high quantum yield and exclusively photo-stable excitation of gold nanoparticles combined with their bio-inert characteristics make them ideal cellular markers. The aim of the study was to visualise gold nanoparticles size-dependently as colloid and in cells after co-incubation. We show the quantification of colloidal gold particles by standard confocal microscopy down to the single particle level. A calibration is demonstrated for pixel numbers in dilution series of uncoated gold nanoparticles. We give implications for practical use of advanced cellular imaging in cultured cells.

Citation Download Citation

Sabine Klein, Svea Petersen, Ulrike Taylor, Detlef Rath, and Stephan Barcikowski "Quantification of colloidal and intracellular gold nanomarkers down to the single particle level using confocal microscopy", Proc. SPIE 7573, Biomedical Applications of Light Scattering IV, 75730L (26 February 2010); https://doi.org/10.1117/12.840985

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