Fiber optic biosensor fabricated for measuring the growth rate of Escherichia coli K-12 in the aqueous

M. I. Zibaii; A. Kazemi; H. Latifi; M. Karimi Azar; S. M. Hosseini; M. H. Ghezelaiagh

doi:10.1117/12.866073

8 September 2010 Fiber optic biosensor fabricated for measuring the growth rate of Escherichia coli K-12 in the aqueous

M. I. Zibaii, A. Kazemi, H. Latifi, M. Karimi Azar, S. M. Hosseini, M. H. Ghezelaiagh

Author Affiliations +

Proceedings Volume 7653, Fourth European Workshop on Optical Fibre Sensors; 765323 (2010) https://doi.org/10.1117/12.866073
Event: (EWOFS'10) Fourth European Workshop on Optical Fibre Sensors, 2010, Porto, Portugal

Abstract

A single-mode tapered fiber optic biosensor was utilized for real-time monitoring of the Escherichia coli (E. coli K-12) growth in an aqueous medium. The applied fiber tapers were fabricated using heat-pulling method with waist diameter and length of 6-7μm and 3mm, respectively. The bacteria were immobilized on the tapered surface using Poly-L-Lysine. By providing the proper condition, bacterial population growth on the tapered surface increases the average surface density of the cells and consequently the refractive index (RI) of the tapered region would increase. The adsorption of the cells on the tapered fiber leads to changes in the optical characteristics of the taper. This affects the evanescent field leading to changes in optical throughput. The bacterial growth rate was monitored at room temperature by transmission of a 1558.17nm distributed feedback (DFB) laser through the tapered fiber. At the same condition, after determining the growth rate of E. coli by means of colony counting method, we compared the results with that obtained from the fiber sensor measurements. This novel sensing method, promises new application such as rapid analysis of the presence of bacteria.

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M. I. Zibaii, A. Kazemi, H. Latifi, M. Karimi Azar, S. M. Hosseini, and M. H. Ghezelaiagh "Fiber optic biosensor fabricated for measuring the growth rate of Escherichia coli K-12 in the aqueous", Proc. SPIE 7653, Fourth European Workshop on Optical Fibre Sensors, 765323 (8 September 2010); https://doi.org/10.1117/12.866073

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