The effects of Ce3+ and Ce4+ on the stability of fibroblast growth factor-2

Liwei Sun; Hao Feng; Rui Jiang; Liping Niu; Yu Song; Kai Feng; Chao Qi

doi:10.1117/12.868223

8 November 2010 The effects of Ce³⁺ and Ce⁴⁺ on the stability of fibroblast growth factor-2

Liwei Sun, Hao Feng, Rui Jiang, Liping Niu, Yu Song, Kai Feng, Chao Qi

Proceedings Volume 7845, Optics in Health Care and Biomedical Optics IV; 784515 (2010) https://doi.org/10.1117/12.868223
Event: Photonics Asia 2010, 2010, Beijing, China

Abstract

The interaction between tri or tetravalent cerium ions and basic fibroblast growth factor (FGF-2) at 0.1-6: 1 molar ratio under physiological condition was studied by fluorescence and CD spectrum. The different spectra alterations of FGF-2 induced by Ce³⁺ and Ce⁴⁺ showed that Ce³⁺ and Ce⁴⁺ caused different conformational changes of FGF-2 respectively, though both of them destabilized the protein. The instability of FGF-2 in the presence of Ce³⁺ is involved in the oxidation of its free cystein of protein, but that this treatment nearly does not affect the biological activity. As to Ce⁴⁺, it not only induced the conformational changes of protein but also inhibits its activity in a dose-dependent manner, which could be relative to the electrostatic repulsion between Ce⁴⁺ and its basic amino acid residues (pI=9.6) or the specific binding of Ce⁴⁺ to deprotonated amino acid residues. The interesting results would be helpful to investigate the problem of the stability of proteins.

Citation Download Citation

Liwei Sun, Hao Feng, Rui Jiang, Liping Niu, Yu Song, Kai Feng, and Chao Qi "The effects of Ce³⁺ and Ce⁴⁺ on the stability of fibroblast growth factor-2", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 784515 (8 November 2010); https://doi.org/10.1117/12.868223

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