Wide-field two-photon microscopy with temporal focusing and HiLo background rejection

Elijah Y. S. Yew; Heejin Choi; Daekeun Kim; Peter T. C. So

doi:10.1117/12.876068

28 February 2011 Wide-field two-photon microscopy with temporal focusing and HiLo background rejection

Elijah Y. S. Yew, Heejin Choi, Daekeun Kim, Peter T. C. So

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 79031O (2011) https://doi.org/10.1117/12.876068
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Scanningless depth-resolved microscopy is achieved through spatial-temporal focusing and has been demonstrated previously. The advantage of this method is that a large area may be imaged without scanning resulting in higher throughput of the imaging system. Because it is a widefield technique, the optical sectioning effect is considerably poorer than with conventional spatial focusing two-photon microscopy. Here we propose wide-field two-photon microscopy based on spatio-temporal focusing and employing background rejection based on the HiLo microscope principle. We demonstrate the effects of applying HiLo microscopy to widefield temporally focused two-photon microscopy.

Citation Download Citation

Elijah Y. S. Yew, Heejin Choi, Daekeun Kim, and Peter T. C. So "Wide-field two-photon microscopy with temporal focusing and HiLo background rejection", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031O (28 February 2011); https://doi.org/10.1117/12.876068

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