High-speed focal modulation microscopy using acousto-optical modulators for visualization of thick biological specimens

Shau Poh Chong; Guangjun Gao; Colin J. R. Sheppard; Nanguang Chen

doi:10.1117/12.873996

11 February 2011 High-speed focal modulation microscopy using acousto-optical modulators for visualization of thick biological specimens

Shau Poh Chong, Guangjun Gao, Colin J. R. Sheppard, Nanguang Chen

Author Affiliations +

Proceedings Volume 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII; 79040C (2011) https://doi.org/10.1117/12.873996
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

In recent development of fluorescence microscopy, the out-of-focus fluorescence background that arises when imaging deep inside biological tissues is critical in determining the image quality and penetration depth. Focal modulation microscopy (FMM) is an advanced fluorescence technique that can provide high subcellular resolution when imaging thick specimens mainly by preserving the signal-to-background ratio.

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Shau Poh Chong, Guangjun Gao, Colin J. R. Sheppard, and Nanguang Chen "High-speed focal modulation microscopy using acousto-optical modulators for visualization of thick biological specimens", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79040C (11 February 2011); https://doi.org/10.1117/12.873996

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KEYWORDS

Luminescence

Modulation

Microscopy

Confocal microscopy

Image quality

Bragg cells

Curium

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