4D confocal microscopy method for drug localization in the skin

Ulf Maeder; Thorsten Bergmann; Jan Michael Burg; Sebastian Beer; Peggy Schlupp; Thomas Schmidts; Johannes T. Heverhagen; Frank Runkel; Martin Fiebich

doi:10.1117/12.889497

8 June 2011 4D confocal microscopy method for drug localization in the skin

Ulf Maeder, Thorsten Bergmann, Jan Michael Burg, Sebastian Beer, Peggy Schlupp, Thomas Schmidts, Johannes T. Heverhagen, Frank Runkel, Martin Fiebich

Author Affiliations +

Proceedings Volume 8086, Advanced Microscopy Techniques II; 80861A (2011) https://doi.org/10.1117/12.889497
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

A 4D confocal microscopy (xyzλ) method for measuring the drug distribution in skin samples after a permeation study is investigated. This approach can be applied to compare different drug carrier systems in pharmaceutical research studies. For the development of this detection scheme phantom permeation studies and preliminary skin measurements are carried out. The phantom studies are used to detect the permeation depth and the localization of the external applied fluorescent dye naphthofluorescein that is used as a model agent. The skin study shows the feasibility of the method for real tissue. For the differentiation of tissue/phantom and the dye, spectral unmixing is performed using the spectral information detected by a confocal microscope. The results show that it is possible to identify and localize external dyes in the phantoms as well as in the skin samples.

Citation Download Citation

Ulf Maeder, Thorsten Bergmann, Jan Michael Burg, Sebastian Beer, Peggy Schlupp, Thomas Schmidts, Johannes T. Heverhagen, Frank Runkel, and Martin Fiebich "4D confocal microscopy method for drug localization in the skin", Proc. SPIE 8086, Advanced Microscopy Techniques II, 80861A (8 June 2011); https://doi.org/10.1117/12.889497

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Skin

Confocal microscopy

Tissues

Signal detection

Luminescence

Microscopes

Scattering

Show All Keywords

Keywords/Phrases

Search In:

Publication Years