Vertical differential interference contrast using SLMs

Michael Warber; Malte Hasler; Tobias Haist; Wolfgang Osten

doi:10.1117/12.889889

8 June 2011 Vertical differential interference contrast using SLMs

Michael Warber, Malte Hasler, Tobias Haist, Wolfgang Osten

Proceedings Volume 8086, Advanced Microscopy Techniques II; 80861E (2011) https://doi.org/10.1117/12.889889
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

In microscopy, several different phase contrast methods (e.g. Zernike contrast, differential interference contrast) have been developed to image phase objects. For these methods specialized microscopic equipment (modified microscope objectives, filters, etc.) is needed. The static elements within such microscopes are a trade-off, because phase contrast imaging depends strongly on the object and the information to be visualized. We show results of an implementation of many different phase contrast methods using a high resolution phase-only spatial light modulator (SLM) in the pupil plane. All implemented methods are realized by software. Therefore, it is not only feasible to change the different phase contrast methods in real time, but it is also possible to optimize the parameters. Images obtained from different settings are combined digitally to improve the final image quality. Furthermore, completely new phase contrast filters can be tested easily because the phase of each pixel can be changed arbitrarily. We use this method to implement a new phase contrast filter that is obtained by combining a focused and a defocused point spread function. We will present theoretical as well as experimental results of this vertical differential interference contrast filter.

Citation Download Citation

Michael Warber, Malte Hasler, Tobias Haist, and Wolfgang Osten "Vertical differential interference contrast using SLMs", Proc. SPIE 8086, Advanced Microscopy Techniques II, 80861E (8 June 2011); https://doi.org/10.1117/12.889889

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